The Single Best Strategy To Use For PP88
The Single Best Strategy To Use For PP88
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By “host modulatory molecule” or “HMM” is meant herein any molecule, made by reported receiver bacterial mobile, that acts, specifically or indirectly, at the extent of the host organism.
wherein, once delivered into stated specific receiver bacterial mobile, said nucleic acid of fascination produces claimed supplied impact on mentioned focused receiver bacterial cell when claimed vector isn't replicated in reported targeted receiver bacterial cell.
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By “modulating the microbiome functionality” is meant herein changing the purpose of unique species or strains of claimed microbiome, by way of example by earning precise species or strains Convey distinct molecules, or by producing certain species or strains end expressing specific molecules.
The existing inventors indeed developed herein a specific conditional origin of replication derived from PICIs.
In conclusion, conditional origins of replication based upon PICIs enable for output at high titers of huge payloads (˜twelve kb) and nuclease-mediated killing of the goal pressure from the absence of collection and primase protein.
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261、细菌噬菌体可选自短尾噬菌体科(非限制性地比如以下属：fri1病毒、kp32病毒、kp34病毒、phikmv病毒、prado病毒、sp6病毒、t7病毒、cp1病毒、p68病毒、phi29病毒、nona33病毒、pocj病毒、tl2011病毒、bcep22病毒、bpp1病毒、cba41病毒、dfl12病毒、ea92病毒、epsilon15病毒、f116病毒、g7c病毒、jwalpha病毒、kf1病毒、kpp25病毒、lit1病毒、luz24病毒、luz7病毒、n4病毒、nonana病毒、p22病毒、web page病毒、phieco32病毒、prtb病毒、sp58病毒、una961病毒和vp5病毒)。
In an alternate embodiment, claimed vector could be a plasmid, a lot more notably a conjugative plasmid, and claimed donor bacterial cell may possibly then certainly be a bacterium that is certainly capable of transferring reported conjugative plasmid to another bacterium, in particular by conjugation.
in a single embodiment, the focused receiver bacteria are Bacteroides thetaiotaomicron and/or Bacteroides faecis.
specially, the level of vectors in accordance with the invention, specially a vector packaged right into a supply motor vehicle in accordance with the invention, preferably a packaged plasmid or phagemid into a bacterial virus particle in accordance with the creation, or of a pharmaceutical or veterinary composition according to the invention, to get administered must be based on typical treatment recognized by People of ordinary expertise within the artwork.
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140、根据本公开的感兴趣的核酸可包含编码cas蛋白的核酸序列。多种crispr酶可用于用作质粒上感兴趣的序列。在一些实施方案中，crispr酶为ii型crispr酶。在一些实施方案中，crispr酶催化dna切割。在一些其他实施方案中，crispr酶催化rna切割。优选地，crispr酶不造成双链断裂。在一些实施方案中，crispr酶造成单链断裂或切口。在一些实施方案中，crispr酶不会在dna或rna中造成任何断裂。在一个实施方案中，cas13-脱氨酶融合被用于对rna进行碱基编辑。
The sequence encoding Mad7 (your complete protein or a fraction thereof) as Employed in the context in the invention is disclosed in Intercontinental software WO2018/236548.
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